Real-time PCR-system Eco 48 | Type : Eco 48
Výrobca: Cole-Parmer Ltd. (PCRmax)
High specification, economically priced real-time thermocycler. The Eco 48 system has a unique high thermal block uniformity (± 0.1°C). This uniformity as well as validated sample volumes of down to 5 µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ±0.1°C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. Eco 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt.
Features:
- Validated volume per well: 5 to 20 µl
- High temperature uniformity of ±0.1°C across the whole block instantly after every temperature change
- 40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized
- Genotype in 4 minutes with over 99% accuracy
- Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation
- Dual LED excitation (452 nm to 486 nm and 542 nm to 582 nm)
- 4 emission filters (505 nm to 545 nm, 562 nm to 596 nm, 604 nm to 644 nm, 665 nm to 705 nm)
- Data is always collected in all four filters for all wells
- Distinguishes 5000 and 10000 template copies with 99 percent confidence
Additional features:
- 48-well block
- MIQE compliant
- 400 analyte specific qPCR reagent kits available
- No calibration required
- Calibrated dyes: SYBR®, FAMTM, HEXTM, ROXTM, Cy®5.
- Additional dyes within the filter wavelength range are supported with no additional calibration required
- Unlimited software license
- 2 year warranty
Custom 48-well qPCR plates and corresponding seals have to be ordered separately.